Composite
phiC31TLU1

Part:BBa_K3254010:Design

Designed by: Yeqing Zong, Li Haoyuan   Group: iGEM19_GENAS_China   (2019-10-10)


Translational Unit for phiC31 integrase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1764
    Illegal SapI.rc site found at 1455
    Illegal SapI.rc site found at 1513
    Illegal SapI.rc site found at 1618


Design Notes

The RiboJ part was added before the RBS-coding sequence, so that the modularity would be improved.

Source

Assembled by this group.

References

Zong, Y., et al., Insulated transcriptional elements enable precise design of genetic circuits. Nat Commun, 2017. 8(1): p. 52.